The TwinBees, who were living peacefully in Donburi Island, receive a challenge from an unknown individual calling himself Dr. Nikki. "How dare you destroy my creation King Spice, as well as Gattlantis and Poko Poko! This time I'll be your opponent. I'll be waiting for you at Puka Puka Island." Dr. Nikki was none other than Dr. Cinnamon's childhood rival. Whereas Cinnamon decided to use his intellect for the greater good, Nikki became a mad scientist. After reading the challenge, TwinBee and WinBee decide to head to Puka Puka Island without Dr. Cinnamon's knowledge, leaving GwinBee in charge of protecting the scientist. Can the TwinBees truly defeat Dr. Nikki?