Otome Senki is set in 16th century, during the Sengoku ("Warring States") period of Japanese history. The plot of the game is rather simple: an unnamed princess is kidnapped by demons, and the warlord Oda Nobunaga calls the bravest warriors to rescue her. However, all the men are either gravely wounded or unwilling to fight. Nine young maidens join forces and venture into a monster-infested dungeon to rescue the princess.